10-12-0101-pr Stem Cell Biology and Epigenetics - Practical Lab Course

Instructors: Prof. Dr. Cristina Cardoso; Dr. of Phil. Henry Herce; Dr. phil. Francesco Natale; Prof. Dr. Ulrike Nuber

Event type: practical / lab /internship

Org-unit: Dept. 10 - Biology

Displayed in timetable as: 10-12-0101-pr

Subject:

Crediting for:

Language of instruction: Englisch

Min. | Max. participants: - | -

Course Contents:
Own projects are developed in small groups and pursued in the following weeks. The projects are located within the field of stem cell biology and epigenetics and focus, e.g., on the following methods:
•          Application of biological databases
•          Plannin/generation of mammalian expression vectors, CRISPR/Cas9 constructs
•          Gene expression analyses and FISH
•          Domain mapping of chromatin factors
•          In vitro protein-protein interactions (Co-immunoprecipitation)
•          In vivo protein protein interactions (Mammalian Two-Hybrid Assay)
•          Cell culture and differentiation
•          Transient and stable transfection of mammalian cells and reporter cell lines
•          Immunofluorescence and live cell microscopy, protein dynamics (FRAP…)

Online Offerings:
all relevant information can be found on Moodle